Composite

Part:BBa_K209481:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-05)


pcDNA-SSFYFP-DOR-LPD (775-1250)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2387
    Illegal PstI site found at 5854
    Illegal PstI site found at 6403
    Illegal PstI site found at 7861
    Illegal PstI site found at 8236
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 952
    Illegal NheI site found at 895
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2387
    Illegal PstI site found at 5854
    Illegal PstI site found at 6403
    Illegal PstI site found at 7861
    Illegal PstI site found at 8236
    Illegal NotI site found at 978
    Illegal NotI site found at 7382
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 952
    Illegal BglII site found at 12
    Illegal BglII site found at 5636
    Illegal BglII site found at 7013
    Illegal BamHI site found at 929
    Illegal XhoI site found at 1059
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2387
    Illegal PstI site found at 5854
    Illegal PstI site found at 6403
    Illegal PstI site found at 7861
    Illegal PstI site found at 8236
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2387
    Illegal PstI site found at 5854
    Illegal PstI site found at 6403
    Illegal PstI site found at 7861
    Illegal PstI site found at 8236
    Illegal NgoMIV site found at 1497
    Illegal NgoMIV site found at 2838
    Illegal NgoMIV site found at 3121
    Illegal AgeI site found at 1013
    Illegal AgeI site found at 7742
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5592
    Illegal BsaI site found at 8206
    Illegal BsaI.rc site found at 882
    Illegal BsaI.rc site found at 4646
    Illegal BsaI.rc site found at 8153
    Illegal BsaI.rc site found at 8220
    Illegal BsaI.rc site found at 8893
    Illegal SapI site found at 3566
    Illegal SapI.rc site found at 2687
    Illegal SapI.rc site found at 2897


Design Notes

Cloned into BBa_K209473.


Source

References